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Corning Life Sciences corning cellbind flasks
Corning Cellbind Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
corning cellbind flasks - by Bioz Stars, 2026-10
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Related Articles

Centrifugation:

Article Title: IL-6 and TGF-β-Secreting Adoptively-Transferred Murine Mesenchymal Stromal Cells Accelerate Healing of Psoriasis-like Skin Inflammation and Upregulate IL-17A and TGF-β
Article Snippet: Upon reaching 80–90% confluency, cells were recovered with a 10-min incubation at 37 °C with 0.13 mL/cm 2 TrypLETM Express Enzyme (Thermo) for detachment. .. Cell debris was removed by centrifugation at 400× g for 5 min, cells were counted using a hemocytometer and were either preserved at 1 × 10 6 cells/mL in Cellbanker cell freezing media (Amsbio) or resuspended into 0.26 mL/cm 2 of culture medium and distributed at a density of 3500 cells/cm 2 in Corning CellBIND flasks. ..

other:

Article Title: Methods for reprogramming cells and uses thereof
Article Snippet: Cells were then transfected using the Amaxa NucleofectorTM 96-well Shuttle Device (Lonza).

Derivative Assay:

Article Title: Senescence and senotherapies in biliary atresia and biliary cirrhosis.
Article Snippet: .. The cells were originally derived from the liver of a male donor aged 4 months as previously described and cultured on Corning CellBIND flasks (Merck, Kenilworth, NJ, USA) [50]. ..

Cell Culture:

Article Title: Senescence and senotherapies in biliary atresia and biliary cirrhosis.
Article Snippet: .. The cells were originally derived from the liver of a male donor aged 4 months as previously described and cultured on Corning CellBIND flasks (Merck, Kenilworth, NJ, USA) [50]. ..



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Schematic representation of experimental designs. All experimental designs commenced with PDL 13 seeded at a density of 3000 cells/cm 2 on Day minus 3 (not shown). (A) Three‐day experiments assessed, in 96‐well plates, the ability of mitomycin C (MMC), sodium arsenite (iAs), or diethanolamine (DEA) to induce micronuclei (MN), which included a 24‐h seeding recovery phase, followed by a 48‐h exposure to media containing test chemicals. (B) Ten‐day experiments investigated the effects of cellular aging and of iAs exposure. Following a 24‐h recovery after seeding, the cells were cultured in <t>T75</t> flasks for 6 days with increasing concentrations of iAs or vehicle control. The cells were then subjected to the same 3‐day protocol without or with MMC. (C) Short‐duration gene expression experiments followed the same protocol as the standard micronucleus experiment, with RNA extraction occurring at the time that MN would be measured. (D) The longer duration gene expression experiments were conducted over 30 days and involved multiple rounds of cell exposures to iAs or vehicle control.
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Schematic representation of experimental designs. All experimental designs commenced with PDL 13 seeded at a density of 3000 cells/cm 2 on Day minus 3 (not shown). (A) Three‐day experiments assessed, in 96‐well plates, the ability of mitomycin C (MMC), sodium arsenite (iAs), or diethanolamine (DEA) to induce micronuclei (MN), which included a 24‐h seeding recovery phase, followed by a 48‐h exposure to media containing test chemicals. (B) Ten‐day experiments investigated the effects of cellular aging and of iAs exposure. Following a 24‐h recovery after seeding, the cells were cultured in <t>T75</t> flasks for 6 days with increasing concentrations of iAs or vehicle control. The cells were then subjected to the same 3‐day protocol without or with MMC. (C) Short‐duration gene expression experiments followed the same protocol as the standard micronucleus experiment, with RNA extraction occurring at the time that MN would be measured. (D) The longer duration gene expression experiments were conducted over 30 days and involved multiple rounds of cell exposures to iAs or vehicle control.
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Schematic representation of experimental designs. All experimental designs commenced with PDL 13 seeded at a density of 3000 cells/cm 2 on Day minus 3 (not shown). (A) Three‐day experiments assessed, in 96‐well plates, the ability of mitomycin C (MMC), sodium arsenite (iAs), or diethanolamine (DEA) to induce micronuclei (MN), which included a 24‐h seeding recovery phase, followed by a 48‐h exposure to media containing test chemicals. (B) Ten‐day experiments investigated the effects of cellular aging and of iAs exposure. Following a 24‐h recovery after seeding, the cells were cultured in <t>T75</t> flasks for 6 days with increasing concentrations of iAs or vehicle control. The cells were then subjected to the same 3‐day protocol without or with MMC. (C) Short‐duration gene expression experiments followed the same protocol as the standard micronucleus experiment, with RNA extraction occurring at the time that MN would be measured. (D) The longer duration gene expression experiments were conducted over 30 days and involved multiple rounds of cell exposures to iAs or vehicle control.
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Schematic representation of experimental designs. All experimental designs commenced with PDL 13 seeded at a density of 3000 cells/cm 2 on Day minus 3 (not shown). (A) Three‐day experiments assessed, in 96‐well plates, the ability of mitomycin C (MMC), sodium arsenite (iAs), or diethanolamine (DEA) to induce micronuclei (MN), which included a 24‐h seeding recovery phase, followed by a 48‐h exposure to media containing test chemicals. (B) Ten‐day experiments investigated the effects of cellular aging and of iAs exposure. Following a 24‐h recovery after seeding, the cells were cultured in T75 flasks for 6 days with increasing concentrations of iAs or vehicle control. The cells were then subjected to the same 3‐day protocol without or with MMC. (C) Short‐duration gene expression experiments followed the same protocol as the standard micronucleus experiment, with RNA extraction occurring at the time that MN would be measured. (D) The longer duration gene expression experiments were conducted over 30 days and involved multiple rounds of cell exposures to iAs or vehicle control.

Journal: Journal of Applied Toxicology

Article Title: Impacts of Inorganic Arsenic Exposure on Genetic Stability of Human Mesenchymal Stromal Cells

doi: 10.1002/jat.4785

Figure Lengend Snippet: Schematic representation of experimental designs. All experimental designs commenced with PDL 13 seeded at a density of 3000 cells/cm 2 on Day minus 3 (not shown). (A) Three‐day experiments assessed, in 96‐well plates, the ability of mitomycin C (MMC), sodium arsenite (iAs), or diethanolamine (DEA) to induce micronuclei (MN), which included a 24‐h seeding recovery phase, followed by a 48‐h exposure to media containing test chemicals. (B) Ten‐day experiments investigated the effects of cellular aging and of iAs exposure. Following a 24‐h recovery after seeding, the cells were cultured in T75 flasks for 6 days with increasing concentrations of iAs or vehicle control. The cells were then subjected to the same 3‐day protocol without or with MMC. (C) Short‐duration gene expression experiments followed the same protocol as the standard micronucleus experiment, with RNA extraction occurring at the time that MN would be measured. (D) The longer duration gene expression experiments were conducted over 30 days and involved multiple rounds of cell exposures to iAs or vehicle control.

Article Snippet: The concentration of live cells was then determined with an automated cell counter and then seeded at a density of 3333 viable cells/cm 2 in CellBIND T75 flasks (Corning) with 10‐mL XR media.

Techniques: Cell Culture, Control, Gene Expression, RNA Extraction